supercritical co2 extraction machine
Home >> Supercritical Fluid System >> Supercritical Fluid Technologies Treatises >> Supercritical CO2 Fluid Extraction and Antioxidant Activity of Ginger Oleoresin

Supercritical CO2 Fluid Extraction and Antioxidant Activity of Ginger Oleoresin


Ginger oleoresin was extracted from dried ginger powder using supercritical CO2 fluid with the addition of absoluteethanol as a colsolvent. The effects of extraction pressure, extraction temperature, extraction time, particle size of material andamount of absolute ethanol on extraction yields of ginger oleoresin and gingerols were investigated by single factor and orthogonalarray methods. The optimum conditions for ginger oleoresin extraction were determined as the follows: particle size of driedmaterial, below 120 mesh; extraction pressure, 35 MPa; extraction temperature, 35 ℃, and extraction time 2.5 h. Under theseconditions, the extraction yield of ginger oleoresin and gingerols reached 6.08% and 2.78%, respectively. Ginger oleoresin couldeffectively scavenge DPPH free radicals in a significant dose-dependent manner. GC-MS analysis indicated that there were 76compounds identified in ginger oleoresin, of which 16 ones might belong to the gingerol family and the relative content was33.95%. These results suggest that ginger oleoresin is rich in antioxidant components as gingerols.

ZHENG Jun-cheng ,GAO Zhao-jian1,LIU Hui ,JIANG Yong-hong ,HE Hai-yang1,WANG Ran1,TANG Shi-rong


Ginger oleoresin is mainly extracted by supercritical CO2 extraction and solvent extraction. Supercritical CO2 extraction technology is favored by researchers because of its simple process, high extraction efficiency, and no solvent residues.

At present, the research on supercritical CO2 extraction of ginger oleoresin is mostly limited to process discussion and composition analysis, but the relationship between function and composition is less discussed. This experiment mainly studies the supercritical CO2 extraction process and antioxidant activity of ginger oleoresin, and analyzes its chemical composition by gas chromatography-mass spectrometry (GC-MS) to explore the relationship between oxidation resistance and composition. It is ginger oil Further research on the resin provides a reference.

Materials and Methods

Materials, reagents and instruments

Ginger purchased from Xuzhou Farmers Market

Vanillin (purity> 99%) Sinopharm Chemical Reagent Co., Ltd .;

Reagents such as absolute ethanol are commercially available in analytical grade and are distilled on a rotary evaporator before use.

HP 6890/5973 GC-MS (equipped with electron bombardment ion source (EI) and MSD ChemStation data processing system) Hewlett-Packard Company, USA;

HA121-50-01 Supercritical Extraction Unit Nantong Huaan Supercritical CO2 Extraction System Co., Ltd .;

TU-1810 Ultraviolet Spectrophotometer Beijing General Analysis Co., Ltd.


The factors affecting the extraction of ginger oleoresin by supercritical CO2 were discussed through single-factor experiments and orthogonal experiments, and the optimal extraction process parameters were determined:

Fresh ginger was dried by far-infrared at 50 ℃, pulverized through a 120 mesh sieve, 35 MPa, and extracted at 35 ℃ for 2.5 h. The mass extraction rate was 6.08%, and the ginger curcumin extraction rate was 2.78%. Antioxidant activity experiments show that the extract has a strong ability to scavenge DPPH free radicals, and the scavenging ability has a clear dose-effect relationship with concentration. GC-MS analysis identified 76 substances, of which low-molecular-weight olefins were easily volatile 56.66% and gingerols were 33.95%, which fully demonstrated the advantages of supercritical CO2 extraction of flavor components.

Supercritical co2 extraction equipment

Related Posts

Technology of Decaffe ination of Green Tea Concentrative Liquid Using Supercritical CO2

Decaffeinating test of green tea concen t rative liqu id w as carried out using supercritical CO2. The effect of concent rative liquid consistence, operating pressure, operating temperature and extracting time on decaffeinat ionrate was investigated ..

Supercritical CO2 extraction of ginger oleoresin

Fresh ginger slices are dried at 50 ° C to 13% water content, crushed to a particle size of less than 2.5mm, 1000g is placed in an extraction kettle, and CO2 with a purity of 99.5% is pressed in by a high-pressure plunger pump at a flow rate of 5m..

Tea seed oil supercritical extraction process

The extraction pressure of SFE is the main factor affecting the extraction efficiency of tea seed oil. The solubility of oil in CO2 increases with the increase of pressure, but the increase of pressure will cause a significant increase in equipment i..

Supercritical carbon dioxide fluid extraction of artepillin C from propolis

Using supercritical carbon dioxide as a solvent, combined with chromatography and adsorption technology, a large number of active components of artepillin C in propolis can be separated and purified, and the biological activity of active components o..

SFE extracts squalene from licorice

Through repeated experiments in this experiment, it was found that under the conditions of extraction pressure of 35 MPa and extraction temperature of 40 ℃, squalene was well extracted and separated

Supercritical cleaning technology principle

The solubility of supercritical CO2 increases with the increase of pressure. Changing the pressure and temperature appropriately will cause the solubility to change within a range of 102 to 103 times. Supercritical CO2 cleaning technology uses this f..