Extraction of Camellia oleifera Polyphenols by supercritical CO2 extraction equipment
Experimental results of extracting tea seed oil from tea seeds by supercritical CO2 fluid extraction:
Extraction temperature: 35 °C
Extraction pressure: 30 MPa
Separation temperature: 55 °C
Separation pressure: 8 MPa
Extraction time: 2 h
Tea seed oil yield: 15.4%
Camellia oleifera Abel belongs to Camellia, also known as Camellia, Camellia oleifera and Camellia oleifera. It is distributed in 18 28 34 North latitude, 100 0 122 East longitude. It has more than 200 varieties. It has more than 2000 years of cultivation history. It is also known as "the world's four woody oil plants" with oil palm, olive and coconut. China is the country with the largest variety of Camellia oleifera and the largest yield of Camellia oleifera seeds in the world. Tea oil is the main product of Camellia oleifera. It has high nutritional value and can be comparable to olive oil. It does not contain erucic acid and Erucic acid. Long-term consumption can improve human immunity, prevent and treat hypertension, cardiovascular diseases, prevent obesity, protect liver and resist cancer, but does not produce carcinogenic aflatoxin. Camellia oleifera has many active functions, which are closely related to its polyphenols. Camellia oleifera polyphenols have many active functions.
Supercritical CO2 extraction process of tea seed oil:
The Camellia oleifera seeds harvested after 5 months were used as raw materials for pretreatment. The Camellia oleifera seeds were baked at 40 C for 48 hours, shelled and crushed, and stored after sieving.
Put 200 g camellia seed powder in 1L extraction kettle. Open the valve of the gas storage tank when the temperature reaches the set value. Balance the pressure of extraction kettle, separation kettle I, separation kettle II and pipeline. Then turn on the CO2 pump to boost the pressure. After the pressures of the three kettles reach the predetermined values, the extraction time is recorded and the extraction process is entered. During the extraction process, the pressure of three autoclaves should be adjusted at any time to match the expected value. When the time reached the set value, the whole extraction process was stopped, and the tea seed oil was collected and recorded.
Four factors affecting supercritical CO2 extraction:
Analysis of four influencing factors in supercritical CO2 extraction process: extraction pressure is the key factor affecting supercritical CO2 fluid extraction. The higher the pressure, the higher the density of supercritical CO2 fluid, the stronger the solubility and the higher the extraction rate. The influence of extraction temperature on supercritical CO2 extraction of camellia seed oil is more complex than that of extraction pressure. The increase of extraction temperature decreases the density of CO2 fluid and the solvation effect, and decreases the solubility of camellia seed oil in CO2 fluid, which results in the decrease of extraction rate. On the other hand, with the increase of temperature, the vapor pressure of camellia seed oil increases, resulting in the increase of solubility and extraction rate. For energy saving and emission reduction requirements, the extraction temperature should not be too high, and the low temperature range should be selected. The longer the extraction time is, the better the mass transfer and heat transfer between solvent and solute is, the higher the content of solute in the solvent is, and the higher the extraction rate is. But the longer the extraction time is, the better the extraction rate is. Tea seed oil is a natural product with complex components and similar properties. The longer the extraction time is, the weaker the dissolution and mutual aid effect between components is. In the process of extraction, the extraction intensity should be increased and the extraction rate should be improved as soon as possible. At the same time, the pressure of separator I has little effect on the extraction rate, but it has a great influence on the composition of tea seed oil. Different separation pressures make the components and their contents different. In practice, different target products can be obtained by adjusting the separation pressure, which also proves that the supercritical CO2 extraction process has target selectivity.
Prospect of supercritical co2 extraction of tea seed oil:
Tea seed oil extracted by supercritical CO2 extraction technology retains natural active ingredients. Supercritical fluid extraction technology can avoid solvent residues in traditional solvent removal process and is more environmentally friendly and healthy. It shows that supercritical CO2 extraction method has broad prospects for tea seed oil extraction.