supercritical co2 extraction machine

SFE CO2 extraction process of Pandanus amaryllifolius Roxb. Leaves

SFE CO2 extraction process of Pandanus amaryllifolius Roxb. Leaves

Laboratory equipment:HA231-50-06

Extraction pressure: 20 MPa

Extraction temperature: 45°C

Separation kettle Ⅰ pressure: 12 MPa

Separation kettle Ⅰ temperature: 45°C

Separation kettle Ⅱ pressure: 12 MPa

Separation kettle Ⅱ temperature: 40°C

Separation kettle Ⅲ pressure: 6 MPa

Separation kettle Ⅲ temperature: 40°C

Extraction time: 60 min

Foreword:

The pandanus leaves are the fresh leaves of Pandanus amaryllifolius Roxb., Which are also known as pandan leaves, sage orchids, and pandan leaves. The place of origin is the Makulu Islands, Indonesia, mainly distributed in tropical and subtropical regions of the eastern hemisphere. It is cultivated in Xinglong Overseas Chinese Farm in Hainan, China, and South China Tropical Crop Research Institute in Yixian County. This genus has different economic uses and is an important cash crop after coconut. It is traditionally used in medicine and cooking.

Konjac seed oil supercritical co2 extraction process
Konjac seed oil supercritical co2 extraction process

Fragrant pandanus is used as a traditional Chinese medicine for diuretic, cardiotonic, anti-diabetic, insect repellent, and treatment of skin diseases; because of its unique aromatic odor, it is often used as a spice, popular in Thailand, Indonesia, Malaysia, the Philippines, Some countries, such as Singapore, add several fresh leaves when steaming rice to make the flavor of the steamed rice more intense and lasting; some use it to prepare salads, make pancakes and pastries to increase the flavor of food, and some make it Side dishes. Others use a blender to crush and extract the juice and use it directly as a seasoning, which is an important plant resource for both medicine and food.

Although Fragrant pandanus has been introduced and cultivated in Hainan Province, China, there are few reports on its utilization. In order to further develop and study the value of volatile components in Fragrant pandanus, it is very necessary The supercritical CO2 extraction process is studied in depth.

Extraction instrument:

HA221-50-06 5L supercritical CO2 extraction device (mainly consisting of an extraction kettle, three separation kettles and a rectification column), which is from the Research Center for Integrated Manufacturing and Process Control Technology of South Medicine of Sun Yat-sen University, and Hua'an Supercritical Extraction Machine Limited Joint manufacturing

 Fragrant Pandanus Pretreatment:

The fresh leaves of Xiangludou are naturally dried in a ventilated place to control different water contents, crushed with a pulverizer, passed through a No. 2 sieve, and set aside.

 Supercritical CO2 extraction:

Supercritical CO2 extraction process: carbon dioxide gas cylinder → extraction kettle → separation kettle Ⅰ → separation kettle Ⅱ → separation kettle Ⅲ → carbon dioxide circulation. The crushed fragrant pandanus leaves are charged into an extraction kettle, and the extraction kettle, the separation kettle, and the storage tank are respectively heated or cooled. When the selected temperature is reached, pressurize the system. When the pressure reaches the selected value, close the CO2 gas cylinder, start the cyclic extraction, and maintain the constant temperature and pressure. After the selected time, the separation kettle Ⅰ, Ⅱ, Ⅲ discharge port discharge. Volatile components and yields of Fragrant Pandanus were collected and the content of squalene in volatile components was determined by high performance liquid chromatography.

Design of Supercritical CO2 Extraction Test:

Single factor trial design

In the process of extracting volatile components from Xiangludou, there are many factors that affect the effect of supercritical CO2 extraction. This paper uses a single factor test method to determine the water content, extraction pressure, extraction temperature, extraction time, separation pressure, separation temperature, and yield. The relationship between the rates, preliminary screening of the appropriate process parameter range. The experimental design of the supercritical CO2 extraction process parameters is shown in Table 1.

Box-Behnken's Central Combined Experimental Design:

Based on the single factor test, this paper selects three main factors, extraction pressure (MPa), extraction temperature (° C), and separation pressure (MPa), to optimize the combination according to the design principle of Box-Behnken's central combined test. See Table 2 for design.Taking the three factors of extraction pressure (MPa), extraction temperature (° C), and separation pressure I (MPa) as independent variables, X1, X2, and X3 are used as the independent variables. High, medium and low levels are calculated according to equation (1). Based on the yield of volatile components and the yield of squalene, a response surface method experiment was performed to screen the optimal process conditions for supercritical extraction of volatile components in Fragrant Pandanus.

χi = (Χi -χ0) / Δχ (1)

The independent variables are encoded, where χi is the encoded value of the independent variable, χi is the true value of the independent variable, χ0 is the true value of the independent variable at the test center point, and ΔX is the step size of the independent variable.

Supercritical CO2 extraction process parameter factor level table

Factor level

Moisture content

(%)

Extraction pressure

(MPa)

Extraction temperature

(℃)

Extraction time

(min)

Separation Ⅰ pressure

(MPa)

Separation Ⅰ pressure

(℃)

Separation Ⅱ Pressure

(MPa)

1

43.50

15

35

30

9

35

8

2

21.16

18

40

60

12

40

10

3

11.94

21

45

90

15

45

12

4

9.21

24

50

120

18

50

14

5

 

27

55

 

 

 

 

Effect of moisture content on extraction effect

Effect of different water content on CO2 supercritical extraction
Effect of different water content on CO2 supercritical extraction

Experimental conditions: accurately weigh 300 g each of pandanus leaves with different water contents, put them into a 5L extraction kettle, CO2 flow rate 30 Kg / h, extraction pressure 21 MPa, extraction temperature 45 ° C, extraction time 60 min, separation pressure 9 MPa, temperature 45 ℃, pressure of separation Ⅱ and separation kettle Ⅲ is 6 MPa, temperature of separation Ⅱ and Ⅲ is 40 ℃, the effect of water content on the yield of volatile components and squalene yield in Xiangludou is shown in Figure 1. 2.

Effect of different moisture content on supercritical CO2 extraction of squalene
Effect of different moisture content on supercritical CO2 extraction of squalene

It can be seen from Figures 1 and 2 that with the decrease of the water content, the yield of volatile components and squalene yield of Xiangludou gradually increase, but the moisture content further decreases. The yield of ene began to decrease; the results showed that the moisture content was between 11% and 12%, and the extraction rate had a large value. Therefore, it was more appropriate to control the moisture content of Fragrant pandanus leaves between 11% and 12%.

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