supercritical co2 extraction machine

Supercritical CO2 extraction of Lepidium meyenii Walp oil

Supercritical CO2 extraction of Lepidium meyenii Walp oil process

Maca granularity:: 40 mesh

Laboratory equipment:HA230-50-06

Extraction pressure: 32 MPa

Extraction temperature: 45°C

Separation kettle Ⅰ pressure: 8 MPa

Separation kettle Ⅰ temperature: 45°C

Separation kettle Ⅱ pressure: 6 MPa

Separation kettle Ⅱ temperature: 40°C

Separation kettle Ⅲ pressure: 6 MPa

Separation kettle Ⅲ temperature: 40°C

95% ethanol entrainer volume (ml) : 1: 2

Extraction time: 120 min

Foreword:

Lepidium meyenii Walp is a one-year or biennial herb of the Brassicaceae genus Lepidium, and enjoys the reputation of "Peru ginseng" and "Peru national treasure". It is native to the Andes mountains above 3500 m in Peru. It grows normally under the unique environment of no fertilizer, lack of oxygen, large temperature difference between day and night, and long-term freezing.

Lepidium meyenii Walp
Lepidium meyenii Walp

For thousands of years, as one of the important food sources of the local indigenous people, it is regarded by the Andean gods as a gift given by the Andean gods because of its rich nutritional value and medicinal effects, especially in enhancing energy and fertility. Precious gift.

Lepidium meyenii Walp Oil
Lepidium meyenii Walp

The health effects of Lepidium meyenii Walp are inseparable from the nutritional ingredients such as protein, amino acids, reducing sugars, minerals (zinc, iron, calcium). However, most researchers believe that macamides, benzylglucosinolate (also known as nasturtium glucosinolate) and their decomposition products, benzyl isothiocyanates, are their main active ingredients and characteristics. substance.

Due to its rich nutritional value and medicinal effects, the research and development of Lepidium meyenii Walp is becoming more and more popular, but the research on Lepidium meyenii Walp has not been thorough enough so far, and the relevant Lepidium meyenii Walp products circulating in the market are relatively basic The quality is still uneven.

Therefore, research on the functional factors of Lepidium meyenii Walp is carried out in order to provide a certain scientific basis for the development of high-quality and high-end Lepidium meyenii Walp health food, which has great application value and far-reaching social significance.

In this paper, Lepidium meyenii Walp fat-soluble fractions were obtained by supercritical carbon dioxide extraction, laying a foundation for further in-depth research of Lepidium meyenii Walp and development of functional factors.

Extraction equipment: HA 221-50-06 supercritical CO2 extraction machine", Nantong Huaan Supercritical Extraction Co., Ltd .;

Extraction process:

HA 221-50-06 supercritical CO2 extraction machine
Lepidium meyenii Walp

CO2 gas cylinder → extraction kettle → extraction kettle I → extraction kettleⅡ → extraction kettleⅢ → CO2 circulation

Weigh 500 g of Maca powder (passed through a 40-mesh sieve), put it into a 5 L extraction kettle, heat the extraction kettle, separation kettle Ⅰ, separation kettle Ⅱ, and separation kettle Ⅲ separately, and start cold-cooling.

Among them, the parameters of SFE-CO2 extraction conditions are set as follows: extraction kettle pressure 32 MPa, temperature 45 ℃, separation kettle Ⅰ pressure 8 MPa, temperature 45 ℃, separation kettle Ⅱ and separation kettle Ⅲ pressure are 6 MPa, temperature are 40 ℃

When the temperature of the extraction kettle, the separation kettle and the cold storage tank all meet the experimental requirements, the CO2 from the CO2 gas cylinder is condensed by the cold storage tank, and then driven into the extraction kettle and the three separation kettles through a high-pressure pump;

When the pressure of the extraction kettle and each of the separation kettles reaches the experimental set value, add the entrainer according to the ratio of the amount of feed (g): 95% ethanol entrainer volume (ml) is 1: 2, and start the cycle timing, total circulation Extraction for 2 h;

NO

Area %

Compound Name

Molecular

Formula

Molecular

Weight

1

0.39

Dimethyl sulfone

C2H6O2S

94

2

9.21

Propane,2•fluoro•2•methyl•

C4H9F

76

3

5.49

Benzaldehyde

C7H6O

106

4

0.61

1,2,3•Butanetriol

C4H10O3

106

5

2.37

Benzyl alcohol

C7H8O

108

6

0.44

2,2•Dimethyl•3•heptanone

C9H18O

142

7

0.32

Benzyl isocyanate

C8H7NO

133

8

1.54

Benzene,1•isocyano•2•meth

C8H7N

117

9

3.73

Benzoic acid

C7H6O2

122

10

0.08

1H•Indene,1•methylene•

C10H8

128

11

0.37

Thiophene,2,3•dihydro•

C4H6S

86

12

0.77

Benzaldehyde,4•methoxy•

C8H8O2

136

13

0.67

Divinyl sulfide

C4H6S

86

14

0.71

Benzeneacetic acid

C8H8O2

136

15

0.43

3•Methoxybenzyl alcohol

C8H10O2

138

16

0.16

1•[1•(2,2•Dichloro

vinylimino)•2,2•dimethylpropyl]•3•(p•tolyl)thiourea

C15H19Cl2N3S

343

17

0.56

Bicyclo[4.4.1]und eca•1,3,5,7,9•pentaene

C11H10

142

18

0.24

Bicyclo[4.4.1]undeca•1,3,5,7,9•pentaene

C11H10

142

19

0.20

Benzenemethanol,à•(1•aminoethyl)•,(R*,S*)•(ñ)•

C9H13NO

151

20

0.53

Benzamidomethylbenzoate

C15H13NO3

255

21

0.18

Cycloheptatrienylium, iodide

C7H7I

218

22

1.18

(3•Methoxyphenyl)acetonitrile

C9H9NO

147

23

0.35

Benzeneacetamide,N•methyl•

C9H11NO

149

24

2.38

N•Benzylformamide

C8H9NO

135

25

23.61

Dimethyl(1E)•N•hydroxyethanimidoylphosphonate

C4H10NO4P

167

26

0.41

Benzene,1-azido-3-methyl-

C7H7N3

133

27

0.65

3•Methoxybenzylamine

C8H11NO

137

28

0.47

Dextroamphetamine

C9H13N

135

29

6.75

Benzenemethanesulfonyl fluoride

C7H7FO2S

174

30

1.31

n•Hexadecanoic acid

C16H32O2

256

31

3.72

Eicosanoic acid,ethyl ester

C22H44O2

340

32

0.52

1,E•11,Z•13•Octadecatriene

C18H32

248

33

0.25

Z,Z,Z•4,6,9•Nonadecatriene

C19H34

262

34

0.23

17•Octadecynoic acid

C18H32O2

280

35

2.30

2•Chloroethyllinoleate

C20H35ClO2

342

36

0.97

9,12,15•Octadecatrienal

C18H30O

262

37

1.08

E•11•Hexadecenoic acid, ethyl ester

C18H34O2

282

 

38

 

6.86

Methyl                         2,3,4•tri•O•acetyl•1•(3•(O•aminophenyl)thioureido)

•1•deoxy•beta•d•glucopyranuronate

 

C20H25N3O9S

 

483

39

1.84

cis•5,8,11,14,17•Eicosapentaenoic acid

C20H30O2

302

40

2.09

N,N'•Bis(Carbobe nzyloxy)•lysine methyl(ester)

C23H28N2O6

428

The supercritical carbon dioxide extraction method has the highest yield of the sample, but in terms of extraction time, the supercritical carbon dioxide method only takes 2 hours. At the same time, from the perspective of rational use of medicinal materials, the supercritical carbon dioxide extraction method can obtain a larger immersion per unit time. The paste yield (0.94%), and the subsequent processing of the sample is simple and only needs to recover ethanol. Therefore, the supercritical CO2 extraction method can obtain the fat-soluble parts of maca to a large extent, reducing the waste of maca resources and further To achieve the purpose of rational use of medicinal resources.

By GC / MS analysis, a total of 153 peaks were isolated from the maca fat-soluble sample obtained by the SFE-CO2 extraction method. Compared with the standard database, 40 compounds were identified, as shown in Table 3. Among them, the amides are N-benzylformamide (2.38%), and the long-chain polyunsaturated alkenes (alkyne or acid) are 1, E-11, Z-13-Octadecatriene (0.52%), Z, Z, Z -4,6,9-Nonadecatriene (0.25%), arachidonic acid (1.84%), glucosinolate and its degradation products are benzyl isocyanate (0.32%), 4-methoxybenzaldehyde (0.77 %), 3-methoxyphenylacetonitrile (1.18%);

The maca fat-soluble sample obtained by supercritical CO2 extraction method not only has a high yield (0.94%), but also requires a shorter extraction time (2 h). GC / MS analysis shows that the maca fat-soluble sample obtained by supercritical CO2 extraction method The active ingredients of the sample not only have many kinds, but also have relatively high content. Therefore, compared with the traditional extraction method, the supercritical CO2 extraction method is more in line with the current green, low-carbon, environmentally sustainable development goals.

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