Supercritical CO2 extraction of Lepidium meyenii Walp oil
Supercritical CO2 extraction of Lepidium meyenii Walp oil process
Maca granularity:: 40 mesh
Extraction pressure: 32 MPa
Extraction temperature: 45°C
Separation kettle Ⅰ pressure: 8 MPa
Separation kettle Ⅰ temperature: 45°C
Separation kettle Ⅱ pressure: 6 MPa
Separation kettle Ⅱ temperature: 40°C
Separation kettle Ⅲ pressure: 6 MPa
Separation kettle Ⅲ temperature: 40°C
95% ethanol entrainer volume (ml) : 1: 2
Extraction time: 120 min
Lepidium meyenii Walp is a one-year or biennial herb of the Brassicaceae genus Lepidium, and enjoys the reputation of "Peru ginseng" and "Peru national treasure". It is native to the Andes mountains above 3500 m in Peru. It grows normally under the unique environment of no fertilizer, lack of oxygen, large temperature difference between day and night, and long-term freezing.
For thousands of years, as one of the important food sources of the local indigenous people, it is regarded by the Andean gods as a gift given by the Andean gods because of its rich nutritional value and medicinal effects, especially in enhancing energy and fertility. Precious gift.
The health effects of Lepidium meyenii Walp are inseparable from the nutritional ingredients such as protein, amino acids, reducing sugars, minerals (zinc, iron, calcium). However, most researchers believe that macamides, benzylglucosinolate (also known as nasturtium glucosinolate) and their decomposition products, benzyl isothiocyanates, are their main active ingredients and characteristics. substance.
Due to its rich nutritional value and medicinal effects, the research and development of Lepidium meyenii Walp is becoming more and more popular, but the research on Lepidium meyenii Walp has not been thorough enough so far, and the relevant Lepidium meyenii Walp products circulating in the market are relatively basic The quality is still uneven.
Therefore, research on the functional factors of Lepidium meyenii Walp is carried out in order to provide a certain scientific basis for the development of high-quality and high-end Lepidium meyenii Walp health food, which has great application value and far-reaching social significance.
In this paper, Lepidium meyenii Walp fat-soluble fractions were obtained by supercritical carbon dioxide extraction, laying a foundation for further in-depth research of Lepidium meyenii Walp and development of functional factors.
Extraction equipment: HA 221-50-06 supercritical CO2 extraction machine", Nantong Huaan Supercritical Extraction Co., Ltd .;
CO2 gas cylinder → extraction kettle → extraction kettle I → extraction kettleⅡ → extraction kettleⅢ → CO2 circulation
Weigh 500 g of Maca powder (passed through a 40-mesh sieve), put it into a 5 L extraction kettle, heat the extraction kettle, separation kettle Ⅰ, separation kettle Ⅱ, and separation kettle Ⅲ separately, and start cold-cooling.
Among them, the parameters of SFE-CO2 extraction conditions are set as follows: extraction kettle pressure 32 MPa, temperature 45 ℃, separation kettle Ⅰ pressure 8 MPa, temperature 45 ℃, separation kettle Ⅱ and separation kettle Ⅲ pressure are 6 MPa, temperature are 40 ℃
When the temperature of the extraction kettle, the separation kettle and the cold storage tank all meet the experimental requirements, the CO2 from the CO2 gas cylinder is condensed by the cold storage tank, and then driven into the extraction kettle and the three separation kettles through a high-pressure pump;
When the pressure of the extraction kettle and each of the separation kettles reaches the experimental set value, add the entrainer according to the ratio of the amount of feed (g): 95% ethanol entrainer volume (ml) is 1: 2, and start the cycle timing, total circulation Extraction for 2 h;
Eicosanoic acid,ethyl ester
E•11•Hexadecenoic acid, ethyl ester
N,N'•Bis(Carbobe nzyloxy)•lysine methyl(ester)
The supercritical carbon dioxide extraction method has the highest yield of the sample, but in terms of extraction time, the supercritical carbon dioxide method only takes 2 hours. At the same time, from the perspective of rational use of medicinal materials, the supercritical carbon dioxide extraction method can obtain a larger immersion per unit time. The paste yield (0.94%), and the subsequent processing of the sample is simple and only needs to recover ethanol. Therefore, the supercritical CO2 extraction method can obtain the fat-soluble parts of maca to a large extent, reducing the waste of maca resources and further To achieve the purpose of rational use of medicinal resources.
By GC / MS analysis, a total of 153 peaks were isolated from the maca fat-soluble sample obtained by the SFE-CO2 extraction method. Compared with the standard database, 40 compounds were identified, as shown in Table 3. Among them, the amides are N-benzylformamide (2.38%), and the long-chain polyunsaturated alkenes (alkyne or acid) are 1, E-11, Z-13-Octadecatriene (0.52%), Z, Z, Z -4,6,9-Nonadecatriene (0.25%), arachidonic acid (1.84%), glucosinolate and its degradation products are benzyl isocyanate (0.32%), 4-methoxybenzaldehyde (0.77 %), 3-methoxyphenylacetonitrile (1.18%);
The maca fat-soluble sample obtained by supercritical CO2 extraction method not only has a high yield (0.94%), but also requires a shorter extraction time (2 h). GC / MS analysis shows that the maca fat-soluble sample obtained by supercritical CO2 extraction method The active ingredients of the sample not only have many kinds, but also have relatively high content. Therefore, compared with the traditional extraction method, the supercritical CO2 extraction method is more in line with the current green, low-carbon, environmentally sustainable development goals.