Supercritical co2 extraction of grape seed oil extraction pressure, temperature, time
The best parameters for extracting grape seed oil using a supercritical co2 extraction machine:
Pulverization degree: 40 mesh
Extraction temperature:35 °C
Extraction time:80 minutes
In the technique of extracting grape seed oil by supercritical CO2 fluid, the pulverization degree extraction pressure temperature time is an important factor affecting the efficiency of extracting grape seed oil.
Grape seed oil contains a large amount of unsaturated fatty acids necessary for the human body. Long-term consumption has the effects of reducing blood pressure, lowering blood fat, softening blood vessels, lowering cholesterol, and preventing cardiovascular and cerebrovascular diseases. Therefore, grape seed oil has a good health care function.
The raw material used for grape seed oil is the easy-to-plant waste of the wine, which will become waste if not used, and will pollute the environment. The use of new technologies for comprehensive utilization can greatly increase the value of grapes. Therefore, it has great development prospects.
Physiological function and application of grape seed oil
Grape seed is a by-product of winemaking. If it is not used, it will not only pollute the environment, but also be a waste of resources, because grape seed also contains 13% to 15% of oil and fat, and unsaturated fatty acid content in grape seed oil. Up to 90%, of which linoleic acid content is as high as 80%, comparable to safflower seed oil. Linoleic acid is an important raw material for the synthesis of peanut dilute acid. Not only arachidonic acid is the main substance for the synthesis of prostaglandins, but also has the functions of preventing thrombosis and expanding blood vessels. In addition, grape seed oil also contains a rich physiological oily ingredients, making it have some physiological functions different from ordinary edible oils. Such as lowering blood pressure, clearing cholesterol, and nourishing brain cells. Now, it is used as a specific nutrient health oil for infants, the elderly, high-altitude workers, and hypertensive patients. In addition, well-processed grape seed oil can also be used as a base for cosmetics and pharmaceuticals.
Characteristics of supercritical CO2 fluid extraction
Characteristics of supercritical CO2 fluid extraction
The supercritical CO2 fluid extraction technology is simple in process and convenient in operation; it overcomes the need of distillation heating in the separation process of solvent extraction, and the oil is easily oxidized and rancid; it also overcomes the advantages of low efficiency of the pressing method, cumbersome refining process, and unsatisfactory oil color. The supercritical CO2 fluid extraction technology uses non-toxic, non-residual CO2 instead of water or organic solvent as the extraction medium, and is extracted under conditions close to room temperature, without solvent residual pollution and no pollution to the environment. The technology reduces the disadvantages of natural materials to heat or chemical instability during processing, and preserves the original flavor and nutrients of natural products.
Step of extracting grape seed oil by supercritical CO2 extraction
1) Accurately weigh the pulverized, certain-grained grape seeds in an extraction vessel at a volume of approximately 60% of the volume of the kettle, then fix the extraction vessel in a heating furnace and close the safety door. Ensure that the safety door does not open at will until the end of the extraction process. Check that each valve on the extractor is off.
2) Connect the main power supply, constant temperature circulation system power supply and air compressor power supply separately to check whether the equipment is working properly.
3) Open the intake valve to allow liquid carbon dioxide to enter the extraction system through the siphon
4) Open the temperature control furnace and exhaust valve temperature control switch respectively, and set the extraction temperature and exhaust valve temperature on the temperature control panel.
5) After the kettle temperature reaches the set value and stabilizes, adjust the system pressure to the set value. Keep the exhaust valve closed and start the static extraction process.
6) After the static extraction is completed, the venting valve is opened to start the dynamic extraction process. Adjust the opening and closing degree of the exhaust valve to control the air release rate of carbon dioxide. The supercritical fluid carrying the product expands throttling through the pressure reducing valve, releases the grape seed oil and becomes normal pressure CO2 gas, and the venting flow rate is measured by the float flow meter.
After the end of the extraction, the extract weight was accurately weighed, and the oil content of the grape seed powder was measured by a Soxhlet extractor, and then the extraction rate of the grape seed oil was calculated.
Factors affecting the extraction of grape seed oil by supercritical CO2 fluid extraction equipment
Effect of pulverization degree on grape seed oil extraction
The particle size of the material has a dual effect on the extraction. On the one hand, the finer the material, the mass transfer area is increased, and the mass transfer distance and mass transfer resistance are reduced, which is beneficial to the extraction; on the other hand, if the material is too fine, it is easily compacted under high pressure, increasing the mass transfer resistance without Conducive to extraction. Due to the high content of grape seed oil, it is generally easy to use about 40 mesh.
Effect of extraction intensity on grape seed oil extraction
Supercritical CO2 fluids have good mass transfer characteristics. Increasing the pressure will not only increase the density of CO2, but also reduce the distance between molecules.
At lower temperatures, the extraction rate of grape seed oil gradually increases with increasing pressure, but when the pressure is too high, the extraction rate increases slowly. Because of the high density of CO2 under high pressure, the compressibility is small, and the increase of pressure has little effect on the solubility of the substance. At the same time, high pressure will increase the investment and operating costs of the equipment. Therefore, it is more appropriate to select 28Mpa for extraction from all aspects.
Effect of extraction temperature on grape seed oil extraction
On the one hand, the elevated temperature increases the diffusion coefficient of the substance, so that the solubility increases, which is beneficial to the extraction; on the other hand, since the extraction temperature is an important factor affecting the density of the supercritical CO2 fluid, when the temperature rises, the CO2 is lowered. The density of the material reduces the solubility of the substance, which is not conducive to extraction; in addition, the increase in temperature also degrades the quality of the product. Therefore, the extraction temperature should not be too high.
At 28Mpa, the extraction rate of grape seed oil decreased with the increase of temperature, but the extraction rate increased slowly at the specified temperature of 35 °C; when the pressure was less than or equal to 28Mpa, the temperature was below 35 °C, and the extraction rate increased with temperature. And rise. For the effect of combined pressure and temperature, the optimum temperature for extraction should be 35 °C.
Effect of extraction time on grape seed oil extraction
The supercritical CO2 fluid extraction process is divided into three stages: the initial stage of extraction, the stage of conversion, and the final stage of extraction. In the initial stage of extraction, the contact time of the substance with CO2 is small, and the amount of material per unit time is less; as the extraction time is extended, the extraction stage is gradually increased; the final stage of extraction is due to the low content of the extracted material in the material. In addition, the amount of extraction per unit time is reduced. The results show that at 35 ° C, 28 Mpa, the extraction rate is gradually increased below 80 minutes; the extraction rate below 80 minutes is close to the level and no longer increases. Therefore, the time for extracting grape seed oil from supercritical CO2 fluid should be 80 minutes.
Prospects for supercritical fluid extraction
Supercritical fluid extraction technology is essential for the modernization of traditional Chinese medicine. It is necessary to shift from simple intermediate raw material extraction to taking into account the development and utilization of new Chinese medicines for compound Chinese medicines, or to improve or secondary development of currently produced famous Chinese patent medicines; to strengthen the application of analytical supercritical fluid extraction or supercritical chromatography in traditional Chinese medicine analysis, Continuous reform of traditional analytical methods; supercritical fluid crystallization technology and preparation of ultrafine particles can be used in the development of new dosage forms of traditional Chinese medicine, and should be strengthened in the application of traditional Chinese medicine preparations to promote the modernization of traditional Chinese medicine preparations.
Free supercritical extraction test
China Jiangsu Nantong Huaan Supercritical CO2 Extraction System Co., Ltd has more than 40 years experience of supercritical fluid technology design andequipment manufacturing and it is the high tech company which is committed to the supercritical fluid technology development and supercritical CO2 fluid extraction equipment manufacturing. China a national torch project undertaking unit. Strict implementation of GMP standards, and has passed European CE standard certification， ISO 9001:2000 International Quality Management System Certification, ISO 14001 Environmental Certification and OHSAS 18001 Occupational Health and Safety Certification.
And we specifically supply for our customers custom laboratory, pilot and industrial scale super critical fluid intelligent extraction, dry, reaction, ultrafine grains and other equipment. Also we provide for the customers processing small experiment and cooperation to jointly develop services. Our company not only provides the super critical CO2 extraction distillation, concentration, purification, process technology and equipment of effective components from natural products but also provide the processing technology and equipment of the removal of residual solvent and pesticide residues in products.
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