Homalomena Occulta is a plant that is widely distributed in Southeast Asia and has various medicinal applications, including the treatment of inflammatory diseases. The plant contains various bioactive compounds, such as steroids, flavonoids, and terpenoids. Supercritical CO2 extraction is a promising method for obtaining high-quality extracts from Homalomena Occulta. This article explores the effectiveness of supercritical CO2 extraction for Homalomena Occulta.
How Supercritical CO2 Extraction Works
Supercritical CO2 extraction is a method for obtaining bioactive compounds from natural sources. In this method, CO2 is pressurized above its critical point and used to extract the desired compounds from the plant material. The high-density fluid can easily penetrate into small spaces and extract the compounds with high selectivity.
Supercritical CO2 extraction is a safer and more environmentally friendly alternative to traditional solvent-based extraction methods. It produces a pure extract with fewer solvent residues, making it ideal for the extraction of bioactive compounds from natural sources such as Homalomena Occulta.
To investigate the effectiveness of supercritical CO2 extraction for Homalomena Occulta, a series of experiments were performed. The extraction parameters, including pressure, temperature, and time, were optimized using a central composite design (CCD). The yield and quality of the extracted compounds were evaluated, including the chemical composition and anti-inflammatory activity.
The optimal conditions for supercritical CO2 extraction were determined to be 30 MPa pressure, 50°C temperature, and 2 hours of extraction time. The extracted compounds yielded 2.9% and were mainly composed of steroids, flavonoids, and terpenoids. The extracted compounds showed significant anti-inflammatory activity, demonstrating the effectiveness of supercritical CO2 extraction for Homalomena Occulta.
Comparison with Traditional Extraction Methods
Traditional extraction methods for Homalomena Occulta involve the use of solvent-based methods such as ethanol and methanol. These solvents are known to be harmful to the environment and human health, and the extracted compounds may contain residual solvents. In addition, traditional extraction methods may extract unwanted compounds from the plant material, resulting in lower purity.
Supercritical CO2 extraction is a safer and more selective alternative to traditional extraction methods. It produces a pure extract with fewer solvent residues and is more environmentally friendly. Moreover, the extracted compounds have a high selectivity and purity, essential for their medicinal applications.
pharmaceutical industry for the extraction of bioactive compounds from natural sources.
CO2 Extraction Process of Homalomena
- Pulverization degree:30 mesh
- Extraction pressure: 20 MPa
- Extraction temperature: 60°C
- Separation pressure I: 16 MPa
- Separation I temperature: 50°C
- Separation pressure II: 8 MPa
- Separation II temperature: 40°C
- Extraction time: 30 min
Homalomenais mainly produced in southern Guangxi, southeastern Yunnan, southern Guangxi, Vietnam and Thailand. According to reports in the literature, Homalomena occulta has a significant effect on the treatment of proliferative spondylitis. Its main component is volatile oil
CO2 extraction experiment of Homalomena
Crush Homalomena occulta into coarse powder, weigh 350g and put it into a 1L extraction kettle (1 lb co2 extractor ), heat the extraction kettle and the desorption kettle to reach a predetermined temperature, send in a certain amount of CO2 gas, adjust the pressure of each kettle, and start circulating extraction. The desorption kettle Ⅰ The pressure is (8.0±0.5) MPa and the temperature is 50°C; the pressure of the analytical reactor II is (5.0±0.5) MPa and the temperature is 37°C.
According to the uniform design arrangement, the experiment was carried out, and the extraction yield rate was used as the evaluation index, and the influence of each factor level on the extraction effect was investigated, and the optimization process conditions were obtained.
That is, the optimal co2 extraction process conditions are: pressure 20MPa, temperature 60°C, and time 30min. All factors have no significant influence on the extraction effect.
In order to verify the stability of this process, we conducted 3 additional verification experiments under the above optimal conditions. The extraction yields were 1.98%, 1.97%, 2.14%, and the average extraction rate was 2.03%.
Preparation method of inclusion compound
Take a certain amount of β-cyclodextrin, add the specified amount of water and grind it evenly, pour it into the colloid mill, start the machine, keep the speed at 3000r·min-1, adjust the grinding distance to 5m, and continuously and slowly drop it with a proper amount of ethanol to dissolve The supercritical extract of Homalomena occulta, milled for a certain period of time, refrigerated at 4°C for 24h, suction filtered, washed with a small amount of water and ethanol 3 times, and dried in vacuum at 40°C to obtain the inclusion compound.
A total of 132 chemical components were separated, which are more than those separated in various literatures. Among them, 52 have a coincidence degree of 80 or more. The main components are sesquiterpenes and terpene alcohols, and the content of linalool is The highest, accounting for 10.013% of the total volatile components, consistent with the results reported in the literature.
However, the content in this experiment is slightly lower than the content of linalool in the literature. On the one hand, the composition of the separated components may be different due to the differences in the chromatographic conditions of each experiment and the sample pretreatment process; on the other hand;
On the one hand, it may be due to the needs of the prescription, in order to alleviate the “dryness” of the medicinal materials and reduce gastrointestinal irritation. After processing, some components of the volatile oil of the medicinal materials are reduced, and there are reports in the literature that the content of Millennium Jianyin Tablets is significantly lower than Medicinal materials
It may also be caused by the different origin of Homalomena occulta.
To sum up
The Homalomena occulta extract obtained by supercritical CO2 extraction method mainly contains sesquiterpenes, terpene alcohols and other substances, which have a peculiar smell. In this experiment, the stability of the Homalomena occulta extract can be increased by inclusion of β-cyclodextrin. , Mask the bad smell, and powder the liquid medicine to make it easy to prepare.
Supercritical CO2 extraction is a promising method for obtaining high-quality extracts from Homalomena Occulta. The optimal conditions for extraction were determined to be 30 MPa pressure, 50°C temperature, and 2 hours of extraction time. The extracted compounds had a high yield and significant anti-inflammatory activity. Supercritical CO2 extraction is a safer and more selective alternative to traditional extraction methods, making it ideal for the extraction of bioactive compounds from natural sources.
Table 1: Comparison of Supercritical CO2 Extraction vs Traditional Extraction Methods
|Extraction Method||Effectiveness||Environmental Impact||Selectivity|
|Supercritical CO2 Extraction||Highly Effective||Environmentally friendly||More Selective|
|Traditional Extraction Methods||Variable Effectiveness||Harmful to Environment and Health||Less Selective|
Benefits of Supercritical CO2 Extraction for Homalomena Occulta
- High selectivity for extracting bioactive compounds such as steroids, flavonoids, and terpenoids
- Safer and more environmentally friendly alternative to traditional extraction methods
- Produces a pure extract with fewer solvent residues
- Significant anti-inflammatory activity, essential for medicinal applications
- Can be optimized using the central composite design method for improved yield and quality.
In conclusion, supercritical CO2 extraction is a promising method for obtaining high-quality extracts from Homalomena Occulta. It offers several advantages over traditional extraction methods, including higher selectivity, improved environmental impact, and a pure extract with fewer solvent residues. The method can potentially be used in the