Using supercritical carbon dioxide as a solvent, combined with chromatography and adsorption technology, a large number of active components of artepillin C in propolis can be separated and purified, and the biological activity of active components of artepillin C can be maintained.
Eliminates the need for complicated solvent removal, concentration, and separation procedures, and no safety concerns such as solvent residues, low production costs, environmental protection, safety, and practical value;
Brazilian propolis is derived from Brazilian bees.
Resin is collected from the Brazilian endemic plant Alecrim and wild rosemary buds, bark and other parts, and then passed through the Brazilian killer bee. The glandular secretions of the maxillary gland, wax gland and other glands are mixed and transformed into a dual-source gelatinous solid with a special aromatic odor, which has a spicy taste and a typical green color.
It contains high levels of flavonoids, and contains the unique component Artepillin-C. It is an antioxidant with strong antibacterial, antiviral, antifungal and antiulcer effects. It is a kind of A health food that boosts the body’s immunity.
Isolating, and purifying the active ingredient of artepillin C in propolis can be separated and purified in large quantities to obtain the active ingredient of artepillin C in propolis.
Not only the production cost is low, but the biological activity of the artepillin C component can be maintained.
Propolis extraction process one
Mix 2Kg of propolis with ethanol at a ratio of 1:10 (W / V) to produce 20L of propolis ethanol mixture, and then remove impurities in the propolis ethanol mixture by centrifugation to obtain a propolis ethanol extract.
Under the operating conditions of operating pressure 30MPa, temperature 60 ° C, supercritical carbon dioxide flow rate 9L / hr and propolis ethanol extract flow rate 3L / hr, the supercritical carbon dioxide fluid and propolis ethanol extract were passed into a chromatography column for separation So that the propolis ethanol extract can separate the waxy, artepillin C, and flavonoid components in the chromatography column, and remove the waxy components of propolis from the bottom of the chromatography column, and from the top of the chromatography column Remove artepillin C and flavonoid ingredients.
Propolis extraction process two
Under the operating conditions of operating pressure of 20MPa, temperature of 60 ° C, and supercritical carbon dioxide flow rate of 9L / hr, the supercritical carbon dioxide fluid was passed through an adsorption column with artepillin C and flavonoid components to separate the components of artepillin C. The adsorption column adsorbs, and the flavonoid component is collected at the bottom of the adsorption column.
Under operating conditions of an operating pressure of 20 MPa, a temperature of 60 ° C, and a flow rate of 60% ethanol of 3 L / hr, ethanol was passed through the adsorption column for purification to desorb the adsorbed artepillin C component at the bottom of the adsorption column. The purified artepillin C fraction was collected at the top of the adsorption column.