According to the results of single factor investigation, it is preliminarily determined that the separation process of piperine in the supercritical CO2 extract of pepper is: dripping absolute ethanol in a constant temperature water bath at 40℃, the dosage is 1:5 (w/v), and then 1℃/ The cooling rate of 5min is reduced to 0℃, and it is placed at 0℃ for 4h to crystallize.
Supercritical CO2 extraction of black pepper oleoresin
The sequence of factors affecting the yield of black pepper essential oil is:
CO2 flow rate>extraction temperature>extraction pressure>extraction time; the best extraction conditions are: CO2 flow rate is 22kg/h, extraction pressure is 34MPa, extraction time is 140 minutes, and extraction temperature is 38°C. Under these conditions, the yield of black pepper essential oil is 7. 02%.